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Storage & Reconstitution

Peptide Storage & Reconstitution Guide

Amino Peptides Ltd | Research Use Only

To ensure the integrity of your research and the stability of our >98% purity peptides, proper handling and storage are critical. Peptides are sensitive to temperature, light, and physical agitation. Following these industry-standard protocols will help maintain the potency of the compounds.

1. Initial Storage (Upon Receipt)

All peptides from Amino Peptides Ltd are shipped in a lyophilized (freeze-dried) state, which significantly increases their stability during transit.

  • Short-Term Storage (Daily/Weekly Use): Store vials in a refrigerator at 2°C to 8°C.
  • Long-Term Storage (Months/Years): Store vials in a freezer at -20°C or -80°C.
  • Protection: Keep vials in their original packaging or a dark box to prevent degradation from UV light. Ensure the seals remain intact until the point of use.

2. The Reconstitution Process

Reconstitution is the process of adding a liquid solvent (diluent) to the lyophilized powder.

Step A: Temperature Equilibration

Before adding any solvent, allow the vial to reach room temperature (approximately 15–20 minutes). This prevents moisture from condensing inside the vial when it is opened, which can reduce the peptide’s shelf life.

Step B: Choosing a Solvent

The choice of solvent depends on the specific peptide’s solubility profile. Common laboratory solvents include:

  • Bacteriostatic Water: Most common for research purposes as it contains a preservative (0.9% benzyl alcohol) to inhibit bacterial growth.
  • Sterile Water / Saline: Suitable for immediate use but lacks antimicrobial properties.
  • Acetic Acid / DMSO: Used for specific hydrophobic (water-resistant) peptides.

Step C: Gentle Mixing

  1. Direct Stream: Do not spray the solvent directly onto the lyophilized powder. Instead, aim the needle at the inside wall of the vial and allow the liquid to slide down slowly.
  2. Dissolving: Do not shake the vial. Peptides are delicate protein chains; vigorous shaking can cause "shearing" and denature the compound.
  3. Swirling: Gently swirl the vial in a circular motion until the powder is completely dissolved and the solution is clear.

3. Post-Reconstitution Storage

Once a peptide is in a liquid state, its rate of degradation increases significantly.

  • Refrigeration: Reconstituted peptides must be stored in a refrigerator at 2°C to 8°C.
  • Lifespan: Depending on the peptide and the solvent used, reconstituted solutions typically remain stable for 8 to 12 weeks if kept refrigerated.
  • Avoid Refreezing: Once a peptide has been reconstituted, it is generally advised not to freeze it again, as the formation of ice crystals can damage the peptide structure.

4. Safety & Disposal

  • Personal Protective Equipment (PPE): Always use appropriate gloves, eye protection, and a lab coat when handling research chemicals.
  • Disposal: Dispose of all needles and vials in accordance with UK hazardous waste and "sharps" regulations.

Disclaimer

Amino Peptides Ltd products are intended strictly for laboratory research and development. These products are not for human or veterinary use. Amino Peptides Ltd is not liable for any damages resulting from improper storage or handling.

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