Product Overview
5-Amino-1MQ (5-amino-1-methylquinolinium) is a selective, membrane-permeable small molecule inhibitor of nicotinamide N-methyltransferase (NNMT). Researchers utilise this compound in laboratory models to investigate cellular energy homeostasis and NAD+ salvage pathways. Elevated NNMT expression often correlates with altered metabolic states; thus, targeted inhibition provides a practical framework for studying adipocyte metabolism and intracellular energy regulation.
Synthesised to analytical standards, this compound exhibits exceptional purity, ensuring consistent experimental replication in diverse in vitro assays. It serves as a vital reagent for scientific investigations requiring precise modulation of cellular enzymatic activity without off-target binding.
Technical Specifications
- Chemical Name: 5-amino-1-methylquinolin-1-ium
- CAS Number: 42464-96-0
- Sequence: Not Applicable
- Molecular Formula: C10H11N2+
- Molecular Weight: 159.21 g/mol
- Target: Nicotinamide N-methyltransferase (NNMT)
- Mechanism: NNMT inhibition, intracellular NAD+ modulation
- Half-Life: Approximately 1.5 - 2 hours (animal models)
- Format: Lyophilised Solid
- Appearance: White to off-white crystalline powder
- Grade: Laboratory / Analytical Grade
- Purity: >99% HPLC Verified
Variant Breakdown
- 5mg Lyophilised Vial
- 10mg Lyophilised Vial
- Closure: Rubber stopper with flip-off aluminium seal.
Research Mechanism (Mechanism of Action)
NNMT normally catalyses the transfer of a methyl group from S-adenosylmethionine (SAM) to nicotinamide, producing 1-methylnicotinamide (1-MNA). This enzymatic action depletes the available intracellular pool of nicotinamide, directly limiting the synthesis of nicotinamide adenine dinucleotide (NAD+). In laboratory research, administering 5-Amino-1MQ competitively inhibits NNMT activity, halting the clearance of nicotinamide.
The preservation of nicotinamide substrate drastically increases the efficiency of the NAD+ salvage pathway. Subsequent elevations in NAD+ levels activate sirtuin 1 (SIRT1), a cellular regulatory enzyme dependent on NAD+ availability. Animal models exposed to 5-Amino-1MQ demonstrate accelerated lipid metabolism and extensive white adipose tissue remodelling, driven by up-regulated SIRT1 activity. Researchers observe a marked increase in metabolic expenditure and mitochondrial biogenesis within treated isolated cell cultures.
Safety, Handling & Reconstitution
For optimal solubility and structural stability, researchers must reconstitute the lyophilised solid using Bacteriostatic Reconstitution Solution (0.9% Benzyl Alcohol). Prior to mixing, allow the vial to reach ambient room temperature to prevent condensation. Inject the solvent gently against the inner glass wall, rotating the vial to achieve complete dissolution without violent agitation.
Store the lyophilised solid in a desiccated environment at -20°C out of direct light. Once reconstituted, solutions require immediate refrigeration between 2°C to 8°C to maintain molecular integrity over subsequent experimental phases. Strictly restricted to Laboratory Research Purposes Only.
Scientific References (External Links)
Nature Chemical Biology: “Small molecule inhibitors of nicotinamide N-methyltransferase” View Study
Biochemical Pharmacology: “Nicotinamide N-methyltransferase: A promising target for treating metabolic disorders” View Study
FASEB Journal: “Nicotinamide N-methyltransferase knockdown protects against diet-induced obesity in mice” View Study
Journal of Biological Chemistry: “Inhibition of nicotinamide N-methyltransferase in vivo regulates cellular energy metabolism” View Study
