CJC-1295 with DAC is a synthetic analogue of growth hormone-releasing hormone (GHRH) engineered for extended duration in laboratory environments. Through advanced peptide synthesis, this compound is modified with a laboratory evaluation Affinity Complex (DAC) that allows it to covalently bind to endogenous albumin. As a premium research chemical, UK laboratories utilise this peptide to study sustained receptor activation and cellular signalling cascades without the rapid degradation typical of native peptides. When examining CJC-1295 with DAC vs No DAC, researchers note that the DAC moiety fundamentally alters the pharmacokinetic profile by shielding the sequence from dipeptidyl peptidase-4 (DPP-4) cleavage. Implementing precise reconstitution protocols is essential to maintain molecular stability during these in-vitro assays. By interacting with the GHRH receptor, the peptide initiates a cascade that promotes cyclic AMP (cAMP) production, providing a robust model for studying somatotropic axis regulation. Further structural details and binding affinities can be explored via PubChem.
Public search data clusters around phrases like 'testosterone booster' and 'TRT or peptides' when indexing this compound. Current literature evaluates these trends purely through the lens of cellular and molecular laboratory models. Published studies focus on receptor binding affinities in isolated cell cultures rather than bodily outcomes. Amino Peptides supplies this compound only as a lyophilised laboratory reagent.
- Growth hormone secretagogue receptor (GHSR) isolated in cell-free binding studies.
- Anterior pituitary somatotroph receptors evaluated in molecular-stability models.
- G-protein coupled receptor (GPCR) signalling nodes mapped in isolated cell cultures.
- Albumin-binding domains targeted via the Drug Affinity Complex (DAC) in synthetic plasma models.
- Initiates intracellular cAMP accumulation in isolated somatotroph lineages.
- Promotes sustained receptor agonism through covalent binding to endogenous albumin in cell-free systems.
- Modulates calcium ion influx pathways during controlled laboratory experiments.
- Demonstrates extended proteolytic stability against dipeptidyl peptidase-4 (DPP-4) degradation in molecular models.
Technical Specifications
Variant Breakdown
- 2mg Vial: Provides a precise, smaller quantity of lyophilised peptide, ideal for preliminary binding assays, laboratory evaluation-response calibration, and short-term cellular studies.
- 5mg Vial: Offers a larger yield of the compound, designed for extensive longitudinal studies requiring consistent, repeated application across multiple cell cultures.
Quality Assurance
- HPLC Analysis: High-performance liquid chromatography is utilised to confirm a purity level exceeding 99%, ensuring no significant synthesis by-products interfere with assays.
- Mass Spectrometry: Confirms the exact molecular weight and structural integrity of the peptide-DAC conjugate to guarantee batch-to-batch consistency.
- Lyophilisation: The peptide is freeze-dried under strict atmospheric controls to preserve its tertiary structure and molecular stability prior to reconstitution.
- Endotoxin Testing: Rigorous screening ensures endotoxin levels remain strictly below 0.5 EU/mg, preventing unwanted immune responses in sensitive cellular models.
Research Mechanism
- Binds directly to the GHRH receptor on the surface of pituitary somatotrophs in cellular models.
- Activates the Gs-alpha protein complex, leading to the stimulation of adenylate cyclase and a subsequent rise in intracellular cAMP levels.
- Promotes the activation of Protein Kinase A (PKA), which phosphorylates specific target proteins to regulate gene transcription and cellular secretion.
- Utilises a maleimidopropionyl linker (the laboratory evaluation Affinity Complex) to covalently bond with serum albumin, effectively shielding the peptide from enzymatic degradation.
- Facilitates sustained receptor agonism, allowing researchers to observe prolonged cellular responses without the need for frequent redosing in the culture medium.
Reconstitution & Storage Data
| Vial Strength | Solvent Added | Resulting Concentration |
| 2mg | 1 mL Bacteriostatic Reconstitution Solution | 2.00 mg/mL |
| 2mg | 2 mL Bacteriostatic Reconstitution Solution | 1.00 mg/mL |
| 5mg | 1 mL Bacteriostatic Reconstitution Solution | 5.00 mg/mL |
| 5mg | 2 mL Bacteriostatic Reconstitution Solution | 2.50 mg/mL |
- Lyophilised (Powder): Store in a standard refrigerator (2-8°C) for up to 3 months. For long-term preservation, freeze at -20°C.
- Reconstituted (Liquid): Store at 2-8°C (Refrigerated).
Scientific References
The Journal of laboratory evaluation Endocrinology & Metabolism: "Prolonged stimulation of growth hormone (GH) and insulin-like growth factor I secretion by CJC-1295, a long-acting analog of GH-releasing hormone" View Study
Endocrinology: "CJC-1295, a long-acting growth hormone-releasing hormone analog, enhances GH secretion in vitro" View Study
Peptides: "Albumin-binding peptides for extended half-life: Mechanisms and applications in peptide synthesis" View Study
Journal of Peptide Science: "Synthesis and characterisation of maleimide-conjugated peptides for targeted cellular delivery" View Study
