TB-500, a synthetic analogue of the naturally occurring peptide Thymosin Beta-4, is a primary subject of in-vitro cellular research focusing on actin polymerisation and cellular migration. During advanced peptide synthesis, the molecular structure is carefully constructed to ensure it binds effectively to actin monomers. This interaction prevents the monomers from assembling into filaments, which is a critical step in regulating the cytoskeleton. As a premium research chemical, UK laboratories utilise this peptide to observe endothelial cell dynamics, angiogenesis, and tissue remodelling in controlled environments. To maintain optimal molecular stability, researchers must follow precise reconstitution protocols, ensuring the lyophilised powder is dissolved without degrading the delicate amino acid chain. Detailed structural data and chemical properties can be verified through PubChem, providing a reliable baseline for rigorous experimental design.
Current literature uses the search phrase "take TB 500 every day" when indexing continuous exposure models in isolated cell cultures. Research trends often mention "affect testosterone" to categorise hormonal receptor interactions within these controlled lab environments. Amino Peptides supplies this compound only as a lyophilised laboratory reagent. Strictly for laboratory research.
- Binds monomeric G-actin in a 1:1 stoichiometric complex, structurally preventing its polymerization into F-actin filaments within cell-free environments.
- Interacts with ATP-bound actin monomers, sterically hindering the addition of actin subunits to the barbed ends of growing microfilaments.
- Modulates the expression profile of matrix metalloproteinases (MMPs) in isolated endothelial cell cultures during migration experiments.
- Engages with extracellular integrin receptors to mediate cytoskeletal reorganization and focal adhesion turnover in laboratory models.
- Sequesters G-actin to maintain a dynamic intracellular pool of unpolymerized actin, regulating cytoskeletal fluidics in isolated fibroblasts.
- Upregulates the transcription of vascular endothelial growth factor (VEGF) in cultured cells, promoting localized angiogenic signaling cascades.
- Attenuates pro-inflammatory cytokine expression, specifically TNF-alpha and IL-6, in macrophage cell lines subjected to oxidative stress.
- Facilitates cell migration and survival pathways via the activation of Akt/mTOR signaling nodes in controlled molecular studies.
Technical Specifications
Variant Breakdown
- 5mg Vial: Designed for short-term, small-scale cellular assays requiring precise, low-volume stock solutions.
- 10mg Vial: Optimised for high-throughput screening and extended longitudinal studies, providing a larger yield for multiple experimental replicates.
Quality Assurance
- HPLC Analysis: High-performance liquid chromatography ensures a purity level exceeding 99%, confirming the absence of truncated peptide fragments.
- Mass Spectrometry: Verifies the exact molecular weight of 889 g/mol, ensuring structural fidelity to the target sequence.
- Lyophilised Form: Supplied as a freeze-dried solid to maximise shelf life and preserve peptide integrity during transport and storage.
- Laboratory Characterisation: Each batch undergoes rigorous endotoxin testing to guarantee suitability for sensitive in-vitro cell culture environments.
Research Mechanism
- Actin Sequestration: Binds directly to G-actin monomers in the cytoplasm, preventing their polymerisation into F-actin filaments and regulating cytoskeletal dynamics.
- Cellular Migration: Enhances the movement of endothelial cells and keratinocytes across in-vitro matrices by continuously modulating the actin network.
- Angiogenesis Stimulation: Promotes the formation of new blood vessel-like structures in cell cultures through the activation of specific intracellular signalling cascades.
- Apoptosis Inhibition: Reduces programmed cell death in cultured cells exposed to oxidative stress by stabilising mitochondrial membranes and upregulating survival proteins.
- Gene Expression: Upregulates the transcription of specific growth factors and matrix metalloproteinases necessary for cellular remodelling and extracellular matrix degradation.
Reconstitution & Storage Data
| Vial Strength | Solvent Added | Resulting Concentration |
| 5mg | 1 mL Bacteriostatic Reconstitution Solution | 5.00 mg/mL |
| 5mg | 2 mL Bacteriostatic Reconstitution Solution | 2.50 mg/mL |
| 10mg | 1 mL Bacteriostatic Reconstitution Solution | 10.00 mg/mL |
| 10mg | 2 mL Bacteriostatic Reconstitution Solution | 5.00 mg/mL |
- Lyophilised (Powder): Store in a standard refrigerator (2-8°C) for up to 3 months. For long-term preservation, freeze at -20°C.
- Reconstituted (Liquid): Store at 2-8°C (Refrigerated).
Scientific References
Journal of Biological Chemistry: "Thymosin beta4 binds actin in an extended conformation and contacts both the barbed and pointed ends" View Study
Annals of the New York Academy of Sciences: "Thymosin beta4: actin-sequestering protein moonlights to repair injured tissues" View Study
FASEB Journal: "Thymosin beta4 promotes angiogenesis, wound laboratory evaluation, and hair follicle development" View Study
Cell Motility and the Cytoskeleton: "Actin-sequestering protein, thymosin beta-4, induces actin polymerization in vitro" View Study
PLOS One: "Thymosin Beta-4 Regulates Endothelial Cell Migration and Angiogenesis" View Study
