KPV is a naturally occurring tripeptide derived from the C-terminal region of alpha-melanocyte-stimulating hormone. In laboratory settings, it is extensively studied for its interactions with cellular signalling cascades, particularly its ability to modulate transcription factors like NF-kB within in-vitro models. As a premium research chemical, UK laboratories utilise this peptide to investigate cellular homeostasis and cellular proliferation pathways in vitro. Advanced peptide synthesis techniques ensure high purity, which is critical for maintaining molecular stability during prolonged cellular assays. To achieve optimal results, researchers must follow precise reconstitution protocols, ensuring the lyophilised substrate is correctly dissolved without degrading its structural integrity. Further structural data can be accessed via PubChem.
Research trends often mention the phrase "healing" or "anti-ageing" when cataloguing KPV in public search data. However, current literature strictly evaluates this peptide within laboratory, cellular, or molecular models. Published studies discussed under this heading observe inflammatory modulation in isolated cell cultures rather than bodily outcomes. Amino Peptides supplies this compound only as a lyophilised laboratory reagent.
- Exhibits high-affinity binding to Melanocortin 1 Receptor (MC1R) in isolated macrophage cell lines, initiating downstream immunomodulatory signalling cascades.
- Interacts with cell-surface transport proteins in intestinal epithelial monolayers (Caco-2 cells), demonstrating receptor-mediated endocytosis in cell-free environments.
- Modulates NF-κB transcription factor translocation pathways via cytosolic receptor engagement in controlled molecular stability experiments.
- Attenuates pro-inflammatory cytokine secretion (TNF-α, IL-6) by inhibiting the phosphorylation of IκB kinase (IKK) in cultured dendritic cells.
- Stabilises intracellular reactive oxygen species (ROS) production in stimulated neutrophils through the upregulation of antioxidant enzyme transcription in lab models.
- Promotes tight junction protein expression (ZO-1 and Claudin-1) in disrupted epithelial cell monolayers, preserving barrier integrity during experimental inflammatory stress.
Technical Specifications
Variant Breakdown
- 5mg Vial: Designed for preliminary in-vitro assays, allowing researchers to establish baseline cellular responses and optimise initial dosing parameters.
- 10mg Vial: Suited for high-throughput screening and extended longitudinal studies, providing a consistent batch for comprehensive molecular analysis.
Quality Assurance
- HPLC Analysis: High-performance liquid chromatography is utilised to confirm a purity level exceeding 99%, ensuring the absence of truncated peptide impurities.
- Mass Spectrometry: Molecular weight and structural sequence are rigorously verified using advanced mass spectrometry techniques.
- Lyophilised Stability: The peptide is supplied as a lyophilised solid, extending its shelf life and preserving its molecular conformation prior to reconstitution.
- Endotoxin Testing: Strict laboratory characterisation guarantees an endotoxin limit of <0.5 EU/mg, preventing unwanted cellular activation in sensitive assays.
Research Mechanism
- Receptor Interaction: KPV interacts with melanocortin receptors, specifically MC1R, expressed on the surface of various cell types, initiating intracellular signalling cascades.
- Transcription Factor Modulation: The peptide translocates into the cell to inhibit the activation and nuclear translocation of NF-kB, a primary regulator of inflammatory gene expression.
- Cytokine Suppression: By downregulating NF-kB pathways, KPV reduces the in-vitro secretion of pro-inflammatory cytokines such as TNF-alpha and IL-8.
- Cellular Homeostasis: It promotes epithelial cell migration and structural integrity in laboratory models, supporting tissue repair mechanisms at a cellular level.
Reconstitution & Storage Data
| Vial Strength | Solvent Added | Resulting Concentration |
| 5mg | 1 mL Bacteriostatic Reconstitution Solution | 5.00 mg/mL |
| 5mg | 2 mL Bacteriostatic Reconstitution Solution | 2.50 mg/mL |
| 10mg | 1 mL Bacteriostatic Reconstitution Solution | 10.00 mg/mL |
| 10mg | 2 mL Bacteriostatic Reconstitution Solution | 5.00 mg/mL |
- Lyophilised (Powder): Store in a standard refrigerator (2-8°C) for up to 3 months. For long-term preservation, freeze at -20°C.
- Reconstituted (Liquid): Store at 2-8°C (Refrigerated).
Scientific References
Journal of Investigative Dermatology: "Alpha-melanocyte-stimulating hormone and its C-terminal tripeptide KPV modulate cellular pathways" View Study
Gastroenterology: "KPV attenuates inflammatory responses in intestinal epithelial cells in vitro" View Study
Peptides: "The tripeptide KPV suppresses NF-kB activation and cytokine expression" View Study
Immunology Letters: "Anti-inflammatory effects of KPV in cellular models of immune activation" View Study
Endocrinology: "Melanocortin peptides in the regulation of cellular homeostasis" View Study
